Figure 4. Redox potentials of zebrafish Cytochrome b5 reductase and cytochromes b5a and b5b.
Panel A, CYB5R; Panel B, CYB5a; Panel C, CYB5b. The plots show the fit of the fraction reduced (as determined from absorbance spectra, open circles) to the Nernst equation (solid lines). The insets show the absorbance changes during the reductive titrations. Peaks at 510nm (CYB5R) or 590nm (CYB5a/b) are due to the mediators.