. Author manuscript; available in PMC: 2019 Nov 29.

Published in final edited form as: Nature. 2019 May 29;570(7762):468–473. doi: 10.1038/s41586-019-1250-z

Extended Data Table 2. HIV-1 Envelope-based proteins.

Table summarizes the modifications of all the Envelope proteins used in this study.

Protein	PNGS		Other modifications
Protein	Deleted	Added	Other modifications
RC1	133, 137, 156	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, MD39*
RC1–4FILL	133, 137, 156	230, 241, 289, 344	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, MD39*
RC1-glycanKO	133, 137, 156, 301, 332	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, H330A, MD39*
RC1-glycanKO-GAIA	133, 137, 156, 301, 332	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, GDIR/GAIA, H330A, MD39*
RC1-GAIA	133, 137, 156	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, GDIR/GAIA, MD39*
11MUTBΔ301	133, 137, 301	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, MD39*
RC1Δ301	133, 137, 156, 301	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, MD39*
RC1Δ332	133, 137, 156, 332		V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, MD39*
11MUTB	133, 137	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, MD39*
10MUT	133, 137	-	V134Y, T135A, N136P, N137F, I138L, T139I, D140N, T320F, Q328M, MD39*
7MUT	133, 137	-	V134Y, T135A, N136P, N137F, I138L, T139I, D140N, MD39*
5MUT	-	-	V134Y, N136P, I138L, D140N, MD39*
BG505	-	-	MD39*
RC1–4fill VLP	133, 137, 156	230, 241, 289, 344	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, Spytag, MD39*
RC1-Avitag	133, 137, 156	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, Avitag, MD39*
RC1-glycanKO-Avitag	133, 137, 156, 301, 332	-	V134Y, T135A, I138L, T139L, D140S, D141N, T320F, Q328M, T415V, H330A, Avitag, MD39*