Table 1.

Degree of hydrolysis, protein contents, and yields of the C. sorokiniana protein isolate, hydrolysates, and peptide fractions

Samples	Abbreviations	Protein content (%)	Yield* (%)	Maximum DH (%)
C. sorokiniana biomass		58.23 ± 0.35
Protein isolate	CSPI	65.08 ± 0.88	4.40 ± 0.75
Pepsin hydrolysate	CSHPe	54.73 ± 0.83a	41.27 ± 5.90a	8.16 ± 0.29a
Bromelain hydrolysate	CSHBr	63.87 ± 0.13b	88.55 ± 6.16b	15.93 ± 0.77b
Thermolysin hydrolysate	CSHTh	84.64 ± 1.51c	92.83 ± 10.12b	18.08 ± 1.13b
Pepsin peptide fraction <5 kDa	HFPe5	65.61 ± 2.26a	17.42†
Pepsin peptide fraction <10 kDa	HFPe10	65.40 ± 5.54a	24.09†
Bromelain peptide fraction <5 kDa	HFBr5	73.84 ± 2.34a	15.65†
Bromelain Peptide Fraction <10 kDa	HFBr10	73.94 ± 3.60a	23.72†
Thermolysin peptide fraction <5 kDa	HFTh5	91.67 ± 1.34b	35.13†
Thermolysin peptide fraction <10 kDa	HFTh10	95.83 ± 2.01b	31.63†

Different superscript letters have significantly different (p < 0.05) mean values.

^*The yield was calculated based on the dry weight of the lyophilized pellets over the dry weight of the raw material and protein isolate used during isolation and hydrolysis.

^†Fractionation was only done once for each fraction of the hydrolysates; thus, no replicate was reported for the yields of the fractions.