Figure 11.

Transient receptor potential ankyrin 1 (TRPA1)-dependent calcium influx in cultured nodose neurons after cold stimulation is enhanced upon chronic stress. (A) Representative traces (top), bath temperature (bottom), and (B) the average values of peak amplitudes of calcium imaging in nodose neurons of naive rats during stimulation with cold temperature (about 12°C) in the absence and presence of A-967079 (5 μM) or N-(3-Aminopropyl)-2-[(3-methylphenyl)methoxy]-N-(2-thienylmethyl) benzamide hydrochloride (AMTB; 10 μM). *P < 0.05 vs baseline, ^#P < 0.05 vs cold (one-way ANOVA followed by Bonferroni post-test comparisons). (C) Representative traces (top), bath temperature (bottom), and (D) the average values of peak amplitudes of calcium imaging in nodose neurons of rats previously subjected to a 10-day water avoidance stress (WAS) or sham stress during cold stimulation. Data are expressed as F/F0 (ratio between basal fluorescence in the first 60 seconds and fluorescence at each time point; n = 40 cells). *P < 0.05, **P < 0.01 (Student’s t test).