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. 2019 Jul 30;25(3):442–460. doi: 10.5056/jnm19014

Figure 5.

Figure 5

Cold-induced pronociception requires transient receptor potential ankyrin 1 (TRPA1) in rats. (A, C) Effect of intraperitoneally (ip) or intra-antrally (ia; 0.5 mL) administered vehicle on visceromotor response (VMR) to colorectal distention (CRD). (B, D) A-967079 (A-96; 6 mg/kg, ip or 6 μg/mL, ia) and HC-030031 (HC-03; 1 mg/mL, ia) attenuated the increase in the VMR to CRD after intra-antral infusion of saline at 0°C. (E) Intra-antral infusion of cinnamaldehyde (1.2 mg/mL, 0.5 mL) increased the VMR to CRD. (F) Intra-antral infusion of L-menthol (4 mg/mL, 0.5 mL) decreased the VMR to CRD. Data are expressed as mean ± SEM of VMR expressed as % of 60 mmHg response in the baseline set of CRD. n = 5–6 naive rats per group. (G, H) Intra-antral infusion of A-96 suppressed cold-induced pronociception in rats previously subjected to a 10-day water avoidance stress (WAS). Data are expressed as mean ± SEM of VMR expressed as % of 60 mmHg response in the baseline set of CRD on day 11. n = 8 per group. *P < 0.05, **P < 0.01 (2-way ANOVA followed by Bonferroni post-test comparisons).