Figure 7.

Chronic stress increases transient receptor potential ankyrin 1 (TRPA1) expression in vagal afferents. (A) Representative western blot bands showing increased TRPA1 protein levels in nodose ganglia of rats subjected to a 10-day water avoidance stress (WAS). (B) Expression levels were quantified using ImageJ software (n = 4). (C) TRPA1 mRNA levels in the antral tissues were increased in stressed rats (n = 8). (D) TRPA1 mRNA levels in T6–10 dorsal root ganglia of stressed rats (n = 8). (E) Effect of WAS on mRNA levels of inflammatory cytokines, nerve growth factor (NGF), and inducible nitric oxide synthase (iNOS) in rat antral tissues (n = 8). *P < 0.05, **P < 0.01 (Student’s t test). GAP-DH, glyceraldehyde-3-phosphate dehydrogenase.