Table 1.
Sequences for the Designed Primers Used in Quantitative Polymerase Chain Reaction Experiments
| Species | Gene | ID | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|---|
| Human | TRPA1 | 8989 | GAGAGTCCTTCCTAGAACCATATCTGA | GAGAGTCCTTCCTAGAACCATATCTGA |
| TRPM8 | 79054 | ACTCAGAAGGCTGAGGTACA | TTCAGTCGGAGTCTCACTCT | |
| GAPDH | 2597 | CTGGGCTACACTGAGCACC | AAGTGGTCGTTGAGGGCAA | |
| Rat | TRPA128 | 312896 | TGCTGAGATCGACGGGAGTG | GGGTATGCCAACTCATTCCTGAAC |
| TRPV129 | 83810 | TCATGGGTGAGACCGTCAACAAG | TCCTTGCCGTCAGGAGTGAA | |
| TNF-α | 24835 | CCCAATCTGTGTCCTTCTA | GCTATCCCCATCTGGTCCCC | |
| IL-1β | 24494 | CTGTGACTCGTGGGATGATG | GGGATTTTGTCGTTGCTTGT | |
| IL-17 | 301289 | TCACAGTTGAATCTCAGCAA | TTTTAAATTATTGAGGCT | |
| IL-6 | 24498 | CCGGAGAGGAGACTTCACAG | ACAGTGCATCATCGCTGTTC | |
| iNOS | 24599 | CCCAGACCCTCACACTCAGAT | GTCCAAGAGAAGTTCCCTGTT | |
| NGF | 310738 | TAGCGTAATGTCCATGTTGT | CCACACACTGACACTGTCA | |
| GAPDH | 24383 | GTCTTCACTACCATGGAGAA | TCATGGATGACCTTGGCC |
TRPA1, transient receptor potential ankyrin 1; TRPM8, transient receptor potential melastatin 8; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TRPV1, transient receptor potential vanilloid 1; TNF-α, tumor necrosis factor-α; IL, interleukin; iNOS, inducible nitric oxide synthase; NGF, nerve growth factor.