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. 2019 Jun 30;12(2):347–359. doi: 10.15283/ijsc19041

Fig. 4.

Fig. 4

MiR-507 suppressed the stemness and oxaliplatin resistance of colorectal cancer. (a) The relative expression levels of miR-507 in HCT116 and HT29 cells transfected with miR-507 mimics (miR-507) or mimics negative control (miR-NC) were detected by qRT-PCR. (b) Overexpression of miR-507 in HCT116 and HT29 cells significantly decreased the capacity to form tumorspheres. (c~e) The levels of CD44, SOX2 and OCT4 were determined by qRT-PCR and western blotting in HCT116 and HT29 cells transfected with miR-507 mimics (miR-507) or mimics negative control (miR-NC). (f) The relative expression levels of miR-507 in oxaliplatin resistant HCT116 (HCT116-OxR) and HT29 (HT29-OxR) cells transfected with miR-507 mimics (miR-507) or mimics negative control (miR-NC) were detected by qRT-PCR. (g~i) Overexpression of miR-507 significantly inhibited CRC-OxR cells growth. Cell viabilities were determined by MTT and colony formation assays. (j) Overexpression of miR-507 increased CRC-OxR cells apoptosis. Cell apoptosis was determined by DNA fragmentation assay. (k) The apoptotic markers p53, Caspase-3, Bcl-2 in oxaliplatin resistant HCT116 (HCT116-OxR) and HT29 (HT29-OxR) cells transfected with miR-507 mimics (miR-507) or mimics negative control (miR-NC) were detected by western blotting. The data represent as mean±SD from three independent experiments. *p<0.05, **p<0.01, ***p<0.001.