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. 2019 Jun 30;12(2):347–359. doi: 10.15283/ijsc19041

Fig. 6.

Fig. 6

Restoration of ELK3 expression abrogated the effects of LINC00525 knockdown. (a, b) Expression levels of ELK3 were determined by qRT-PCR and western blot in the parental HCT116 cells. (c) The capacity to form tumorspheres of these parental HCT116 cells was determined by tumorsphere formation assay. (d, e) mRNA and protein levels of CD44, SOX2 and OCT4 in these parental HCT116 cells were determined by qRT-PCR and western blotting. (f~j) Oxaliplatin resistant HCT116 (HCT116-OxR) cells subjected to co-transfection with empty plasmids (sh-CTR+pSin-vec), LINC00525 shRNA and empty plasmid (sh-LINC00525-1+pSin-vec) or LINC00525 shRNA and ELK3 overexpression plasmid (sh-LINC00525-1+pSin-ELK3). (f, g) Expression levels of ELK3 were determined by qRT-PCR and western blotting in the oxaliplatin resistant HCT116 (HCT116-OxR) cells. (h, i) MTT and colony formation assays were used to determine cell growth of the oxaliplatin resistant HCT116 (HCT116-OxR) cells. (j) Apoptosis levels of these oxaliplatin resistant HCT116 (HCT116-OxR) cells were measured by DNA fragmentation assay. (k) The apoptotic markers p53, Caspase-3, Bcl-2 in these oxaliplatin resistant HCT116 (HCT116-OxR) cells were detected by western blotting. The Data represent as mean±SD from three or more independent experiments. *p<0.05, **p<0.01, ***p<0.001.