Table 1.

Differentiation inducers and conditions

Inducer	Working concentration	Induction time	Differentiation medium	Reference for the applied concentration
Decitabine	10 μM	24 h, then medium without inducer every 3 days	BMa+horse serum	3, 4
Angiotensin II	0.1 μM	The whole differentiation period, every 3 days	BM	7
Angiotensin II	1 μM	The whole differentiation period, every 3 days	BM	19
TGF-β1	5 ng/ml	The whole differentiation period, every 3 days	BM	10, 13
TGF-β1	10 ng/ml	The whole differentiation period, every 3 days	BM	11

BM^a: basal differentiation medium; DMEM-low glucose, 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco, Thermo Fisher Scientific, NY, USA).