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. 2019 Jun 20;44(3):995–1005. doi: 10.3892/ijmm.2019.4250

Figure 5.

Figure 5

Regulation of SIRT1 on NF-κB and KLF4 following ABCD1 or HSD17B4 silencing at TNF-α treated condition. (A) Representative western blot images as well as (B) intensity quantification of p-IκBα, nuclear NF-κB and nuclear KLF4 in ABCD1 or HSD17B4 silenced HBMECs with or without sirtinol (20 µM) treatment at TNF-α (10 ng/ml) treated condition. (C) Representative western blot images as well as (D) intensity quantification of p-IκBα, nuclear NF-κB and nuclear KLF4 in ABCD1 or HSD17B4 silenced HBMECs with or without resveratrol (20 µM) treatment at TNF-α (10 ng/ml) treated condition. **P<0.01 and ***P<0.001 vs. the NT control group. #P<0.05 and ##P<0.05. NT, non-targeting; ABCD, ATP binding cassette subfamily D member 1; HBMECs, human brain microvascular endothelial cells; TNF, tumor necrosis factor; NF, nuclear factor; si, small interfering; KLF4, Krüppel-like factor 4; p-IκBα, phosphorylated-inhibitor of NF-κB; NT, non-targeting.