Figure 10.
Effect of hydrogen on cytokine levels and apoptosis induced by H/R in H9C2 cells. Prior to the experiment, scramble control siRNA and PINK1-specific siRNA were transfected into H9C2 cells. Myocardial injury and hydrogen treatment were described in Fig. 6. At 24 h following reoxygenation, cells were harvested to detect the levels of (A) TNF-α, (B) IL-1β, (C) IL-6 and (D) HMGB1 by ELISA. (E) The expression levels of cleaved caspase-3 and (F) the Bcl-2/Bax ratio were detected by western blotting. Values are presented as the mean ± standard error of the mean (n=6). *P<0.05 vs. control group; #P<0.05 vs. H/R group; and $P<0.05 vs. H/R+H2 group. H/R, hypoxia/reoxygenation; siRNA, small interfering RNA; PINK1, PTEN-induced kinase 1; TNF, tumor necrosis factor; IL, interleukin; HMGB1, high mobility group box 1; Con, control.