Skip to main content
. 2019 Jul 8;44(3):913–926. doi: 10.3892/ijmm.2019.4269

Figure 4.

Figure 4

Effect of LS on pit formation and F-actin ring. (A) Inhibition of osteoclastic bone resorption by LS was assessed in osteo-coated plates (×100 magnification; scale bar, 200 µm). (B) The area of resorptive pits was measured using ImageJ software. (C) RANKL-induced F-actin were stained using a fluorescent phalloidin (×100 magnification; scale bar, 200 µm). (D) The number of F-actin rings was measured using ImageJ software. The results are presented as the means ± standard error of the mean (n=3). ##P<0.01 vs. normal; **P<0.01 vs. RANKL-induced control. LS, Leonurus sibiricus L.; F-actin, filamentous actin; RANKL, receptor activator of nuclear factor κ-B ligand.