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. 2019 Jul 16;44(3):885–892. doi: 10.3892/ijmm.2019.4276

Figure 1.

Figure 1

HMGB1 enhances mechanical stress-induced cardiomyocyte hypertrophy. H9c2 cells were transfected with a GFP-expressing plasmid, and 48 h later, they were stimulated with 100 ng/ml HMGB1 and/or mechanical stretch for 24 h. (A) Western blot analysis confirmed successful GFP transfection in H9c2 cells. (B) Positive staining was observed under a fluorescence microscope (scale bar, 10 µm). (C) Quantification of cell surface area. Values are expressed as the mean ± standard error from three independent experiments. **P<0.01 vs. control; #P<0.05 vs. mechanical stretch. HMGB1, high mobility group box-1; GFP, green fluorescence protein.