Skip to main content
. 2019 Jul 16;44(3):885–892. doi: 10.3892/ijmm.2019.4276

Figure 3.

Figure 3

Mechanical stress and HMGB1 stimulation activate JAK2/STAT3 in cardiomyocytes. HMGB1 (100 ng/ml) and/or mechanical stress were applied to cultured cardiomyocytes for 5, 10, 30 or 60 min. Protein expression levels were then analyzed by western blotting. (A) Representative images and (B) quantification of p-JAK2 and t-JAK2 levels. (C) Representative images and (D) quantification of p-STAT3 and t-STAT3 levels. Values are expressed as the mean ± standard error from three independent experiments. *P<0.05 vs. control; #P<0.05 vs. groups with the same stimuli at 5 min. HMGB1, high mobility group box-1; JAK2, Janus kinase 2; p-, phosphorylated; t-, total.