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. 2019 Jul 12;44(3):982–994. doi: 10.3892/ijmm.2019.4275

Figure 5.

Figure 5

The Axl/PI3K/Akt pathway is involved in suppression of alterations in nuclear morphology, attenuation of Bax expression and caspase-3 activation, and enhancement of Bcl-2 by Gas6 in LPS-stimulated H9C2 cardiomyocytes. After pretreatment with TP-0903 or Wortmannin for 15 min, cells were incubated with Gas6 for 2 h, followed by treatment with LPS for (A) 24 h or (B-E) 12 h. (A) Cells were harvested for Hoechst staining (scale bar, 50 µm), (B) caspase-3 activity determination and (C-E) western blotting. (C) Representative western blots and (D and E) semi-quantification of Bax and Bcl-2. Data are presented as the mean ± standard deviation. **P<0.01 vs. the control group; ##P<0.01 vs. the LPS group; $P<0.05, $$P<0.01 vs. the LPS + Gas6 group. Gas6, growth arrest-specific 6; LPS, lipopolysaccharide.