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. 2019 Jul 12;44(3):982–994. doi: 10.3892/ijmm.2019.4275

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Inhibition of NF-κB signaling by Gas6 is Axl/PI3K/Akt-dependent in LPS-treated H9C2 cells. After pretreatment with TP-0903 or Wortmannin for 15 min, the cells were incubated with Gas6 for 2 h, followed by treatment with LPS for the appropriate times. Following LPS administration, H9C2 cells were harvested for analysis. (A and B) Representative western blots and (C and D) semi-quantification of p-IκBα, IκBα, p-P65 and P65. (E-H) Semi-quantification of p-IκBα, IκBα, p-P65 and P65. (I) Translocation of NF-κB P65 was visualized by immunofluorescence analysis (scale bar, 50 µm). Data are presented as the mean ± standard deviation. ^*P<0.05, ^**P<0.01 vs. the control group; ^#P<0.05, ^##P<0.01 vs. the LPS group; ^$P<0.05, ^$$P<0.01 vs. the LPS + Gas6 group. Gas6, growth arrest-specific 6; LPS, lipopolysaccharide; NF-κB, nuclear factor-κB; p-, phosphorylated.

Inhibition of NF-κB signaling by Gas6 is Axl/PI3K/Akt-dependent in LPS-treated H9C2 cells. After pretreatment with TP-0903 or Wortmannin for 15 min, the cells were incubated with Gas6 for 2 h, followed by treatment with LPS for the appropriate times. Following LPS administration, H9C2 cells were harvested for analysis. (A and B) Representative western blots and (C and D) semi-quantification of p-IκBα, IκBα, p-P65 and P65. (E-H) Semi-quantification of p-IκBα, IκBα, p-P65 and P65. (I) Translocation of NF-κB P65 was visualized by immunofluorescence analysis (scale bar, 50 µm). Data are presented as the mean ± standard deviation. ^*P<0.05, ^**P<0.01 vs. the control group; ^#P<0.05, ^##P<0.01 vs. the LPS group; ^$P<0.05, ^$$P<0.01 vs. the LPS + Gas6 group. Gas6, growth arrest-specific 6; LPS, lipopolysaccharide; NF-κB, nuclear factor-κB; p-, phosphorylated.