Figure 3.

The mRNA levels of Nppb, Npr1, Grp and Grpr in the lower cervical spinal cord (C5-8) in the 3 groups. The gene expression levels of Nppb and Grp were significantly increased in the mice with AEW- and DCP-induced chronic itch, respectively. The mRNA expression levels of their receptors, Npr1 and Grpr, were increased in both groups of chronic itch. Each value represents the mean ± SEM of 3 independent experiments (n=5-7 mice per group). ^*P<0.05 and ^**P<0.01 vs. naïve mice. AEW, acetone/ether/water; DCP, diphenylcyclopropenone.