Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 2;44(3):1078–1090. doi: 10.3892/ijmm.2019.4262

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

VEGF, VEGFR2, p-PLCγ1, PLCγ1 and eNOS protein expression in bEnd.3 endothelial cells. (A) Immunofluorescence staining of VEGF (green) in bEnd.3 endothelial cells. Co-localization with the endothelial cell marker CD31 (red) was observed. (B) Immunofluorescence staining of VEGFR2 in bEnd.3 endothelial cells. Scale bar, 20 µm. (C) Fluorescence intensity changes of VEGF protein and (D) VEGFR2 protein in each experimental group. ^§P<0.05 vs. OGD; and ^*P<0.05 vs. control. (E) Representative images from western blot analysis for the proteins VEGF (21 and 42 kDa), VEGFR2 (154 kDa), p-PLCγ1 (155 kDa), PLCγ1 (155 kDa), eNOS (140 kDa) and β-actin (43 kDa). (F) Quantification of western blotting results in each group. ^#P<0.05 vs. OGD; and ^*P<0.05 vs. control. VEGF, vascular endothelial growth factor; VEGFR2, vascular endothelial growth factor receptor 2; p-, phosphorylated; PLCγ1, phospholipase C γ1; eNOS, endothelial nitric oxide synthase; OGD, oxygen-glucose deprivation; HS, hypertonic saline; Con, control.

VEGF, VEGFR2, p-PLCγ1, PLCγ1 and eNOS protein expression in bEnd.3 endothelial cells. (A) Immunofluorescence staining of VEGF (green) in bEnd.3 endothelial cells. Co-localization with the endothelial cell marker CD31 (red) was observed. (B) Immunofluorescence staining of VEGFR2 in bEnd.3 endothelial cells. Scale bar, 20 µm. (C) Fluorescence intensity changes of VEGF protein and (D) VEGFR2 protein in each experimental group. ^§P<0.05 vs. OGD; and ^*P<0.05 vs. control. (E) Representative images from western blot analysis for the proteins VEGF (21 and 42 kDa), VEGFR2 (154 kDa), p-PLCγ1 (155 kDa), PLCγ1 (155 kDa), eNOS (140 kDa) and β-actin (43 kDa). (F) Quantification of western blotting results in each group. ^#P<0.05 vs. OGD; and ^*P<0.05 vs. control. VEGF, vascular endothelial growth factor; VEGFR2, vascular endothelial growth factor receptor 2; p-, phosphorylated; PLCγ1, phospholipase C γ1; eNOS, endothelial nitric oxide synthase; OGD, oxygen-glucose deprivation; HS, hypertonic saline; Con, control.