Effect of high glucose and AMD3100 on migration and invasion ability of circulating fibrocytes. (A) Number of cells in the bottom of the polycarbonate membrane after intervention with different glucose concentration DMEM. (B) Number of adherent cells through the polycarbonate membrane after intervention with different glucose concentration DMEM. (C) Statistical diagram of total cell number after intervention with different glucose concentration DMEM (total cell number=cells in the bottom of the polycarbonate membrane + adherent cells through the polycarbonate membrane); 30 mmol/l glucose vs. 5.5 mmol/l glucose, 30 mmol/l glucose vs. 30 mmol/l glucose+SDF-1, 30 mmol/l glucose+SDF-1 vs. 30 mmol/l glucose+SDF-1+AMD3100, 30 mmol/l glucose vs. 30 mmol/l glucose+AMD3100. (D) Number of transmembrane cells induced by SDF-1. **P<0.01. DMEM, Dulbecco's modified Eagle's medium; SDF-1, stromal cell-derived factor 1; ns, not significant.