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. 2019 Jul 15;15(7):e1007920. doi: 10.1371/journal.ppat.1007920

Fig 2. Antigenicity profile of purified full-length AMC011 Env trimer determined by BLI.

Fig 2

(A) The antigenicity of full-length and SOSIP.664 Env trimers was assessed by BLI (Octet). Bars represent the maximum values of the binding curves (S2 Fig). A panel of 16 bNAbs and 5 non-NAbs was tested: apex directed antibodies (green), V3-glycan (blue), CD4 binding site (black), gp120-gp41 interface (magenta), MPER (brown) and non-NAbs (red). ND: not determined (the lower yields of full-length AMC011 Env in complex with PGT145 precluded a full analysis). The values are representative of two independent experiments. The curves from one representative experiment are shown in S2 Fig. (B) The binding of bNAbs and non-NAbs to full-length and to SOSIP.664 Env trimers, assessed by BLI, was compared. The Spearman correlation (inset) is shown. For BLI values in which both constructs report data, the average of both was used. (C) Same analysis as in (B), but excluding the non-NAbs and MPER-directed NAb. Antibodies are colored as in panel (A).