Fig 3. Antigenicity profile of the full-length Env protein present on the cell membrane or on the virus.

(A) Neutralization of the AMC011 virus was assessed as a proxy for antibody binding to the functional AMC011 trimer on the virus. IC₅₀ and maximum percentage neutralization (MPN) values for a panel of bNAbs and non-NAbs against AMC011 virus are shown. Binding of bNAbs and non-NAbs to the Env protein embedded in the membrane of HEK293F cells was determined by FACS. Half maximal binding concentrations (EC₅₀) and area under the curve (AUC) values are shown. Data are representative for two to four different experiments. (B) Comparison of AMC011 antigenicity obtained by FACS (membrane-bound Env) and neutralization assays (virus-associated functional Env), by FACS and BLI (purified membrane-derived Env) (C) and by neutralization assays and BLI (D) are shown.