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. 2019 Jul 15;15(7):e1007944. doi: 10.1371/journal.ppat.1007944

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© 2019 Jones et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — Surface plasmon resonance (SPR) sensorgrams of AM22, D25, and RSD5-GL Fabs binding to prefusion RSV F from (A) subtype A (strain A2) or (B) subtype B (strain B9320). The raw data is plotted as a black line and the fitted curve used to calculate the binding kinetics is plotted as a thicker red line. Each binding curve has a 180 second association phase, followed by a 500 second dissociation phase. The equilibrium dissociation constant (K_D) is displayed immediately above the SPR curve for each Fab. (C) A table of the association rate constants (k_a) and dissociation rate constants (k_d) determined for the binding kinetics of each Fab. A value of <1 x 10⁻⁵ was used as the lower limit for the dissociation rate constant (k_d) that can be accurately measured by a Biacore X100.