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. 2019 Jul 25;14(7):e0220262. doi: 10.1371/journal.pone.0220262

Fig 1. Time- and dose-dependent IL-1β-induced IL-6 protein release and mRNA expression in canine dermal fibroblasts.

Fig 1

The cells were incubated with (closed circle) or without (open circle) 200 pM IL-1β for indicated times (a, c) or with indicated concentrations of IL-1β for 24 h (b) or 6 h (d). At the end of the incubation, protein release and mRNA expression of IL-6 were detected by ELISA and real-time RT-PCR, respectively. TBP was used as an internal standard. Values are expressed as the mean ± SE of 3 independent experiments. *P < 0.05, compared with 0 h (a, c), 0 pM (b, d).