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. 2019 Jul 25;14(7):e0220262. doi: 10.1371/journal.pone.0220262

Fig 2. Inhibitory effect of ERK1/2 and MEK inhibitors on IL-1β-induced IL-6 mRNA expression and release.

Fig 2

After the pretreatment without (control) or with FR180204 (25 μM), SKF86002 (20 μM), SP600125 (10 μM) and U0126 (10 μM) (ERK1/2, p38 MAPK, JNK and MEK inhibitors, respectively) for 1 h, and then fibroblasts were stimulated with 100 pM IL-1β for 6 h (a) or 24 h (b). ERK1/2 and MEK inhibitors attenuated IL-1β-induced IL-6 mRNA expression (a) and protein release (b) but not p38 MAPK and JNK inhibitors. TBP was used as an internal standard (a). Values are expressed as the mean ± SE of 3 independent experiments. *P < 0.05, compared with control.