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. 2019 Jul 25;14(7):e0220262. doi: 10.1371/journal.pone.0220262

Fig 4. Attenuation of IL-1β-induced IL-6 mRNA expression in canine dermal fibroblasts transfected with ERK1 and ERK2 siRNAs.

Fig 4

(a-c) In fibroblasts transfected with ERK1, ERK2, and scrambled siRNAs, expression of t-ERK1, t-ERK2, and β-actin was detected by western blotting. ERK1 or ERK2 siRNA transfection decreased the expression of ERK1 or ERK2, respectively, while scrambled siRNA transfection did not influence their expression. β-actin was used as an internal standard. Representative results (a) and relative density of ERK1 or ERK2 protein expression in siRNA-transfected cells compared with those in scrambled siRNA-transfected cells (b, c) are illustrated. (d) After transfection with ERK1, ERK2 and scrambled siRNAs, fibroblasts were incubated with or without 100 pM IL-1β for 6 h. At the end of the incubation, IL-6 mRNA expression was determined. TBP was used as an internal standard. ERK1 and ERK2 siRNA transfection reduced IL-1β-induced IL-6 mRNA expression while scrambled siRNA-transfection did not. IL-1β-induced IL-6 mRNA expression was also reduced in ERK1 and 2 double knockdown cells. Values are expressed as the mean ± SE of 3 independent experiments. *P < 0.05.