Table 2. Summary of West Nile virus infectious cDNA clone derived glycosylation mutant viruses that were generated.
| Parental Virusα | Nucleotide Substitutions made to parental WNV.ICβ | Rescued WNV Glycosylation Mutant Virus | Amino Acid Motif | Amino Acid Translationβ | Predicted glycosylation status |
|---|---|---|---|---|---|
| WNV.IC (NYS) | AAT-TAC-TCC | WNV.IC | NYS | Asn- Tyr- Ser | (+) |
| AAC-TAC-ACC | WNV.NYT.IC | NYT | Asn- Tyr- Thr | (+) | |
| AAC-TAC-CCC | WNV.NYP.IC | NYP | Asn- Tyr- Pro | (-) | |
| AAT-TAC-TTC | WNV.NYF.IC | NYF | Asn- Tyr- Phe | (-) | |
| AGC-TAC-TCC | WNV.SYS.IC | SYS | Ser- Tyr- Ser | (-) | |
| AGC-TAC-CCC | WNV.SYP.IC | SYP | Ser- Tyr- Pro | (-) | |
| AAA-TAC-TCC | WNV.KYS.IC | KYS | Lys- Tyr- Ser | (-) | |
| AAT-TAC-TCC | WNV.A’DEL.IC | A’DEL | (-) |
α The N-linked NYS (AAT-TAC-TCC) glycosylation motif of the parental WNV.IC envelope protein was mutagenized to generate seven new WNV glycosylation mutant viruses that are described in the third column.
β Nucleotides and/or amino acids that were substituted are highlighted in bold and underlined.