Fig 3. DAN2 disrupts the integrity of E.coli cell membrane.

The DNA binding dye propidium iodide (PI) was used to evaluate cell membrane permeability of E. coli ATCC 25922 via flow cytometry. 2.0 × 10⁶ CFU/ml was incubated with varying concentrations of peptide for an hour and PI added subsequently. Flow cytometry was performed using a FACScan instrument. (A) Bacteria; (B) Heat treated (positive control); (C) DAN2 (48 μg/ml); (D) DAN2 (36 μg/ml); (E) DAN2 (24 μg/ml). Bacterial cells treated with either peptide or heat shocked have increased cellular fluorescence intensity of PI.