Figure 3. mGluR-Mediated Excitatory Conductance Drives Burst Firing in ChIs.

(A) Schematic of the recording condition. Cell-attached and whole-cell voltage-clamp recordings were sequentially made from the same ChI.

(B) Representative traces from the same ChI under cell-attached mode (top) and voltage-clamp mode (bottom) following dopamine photoactivation.

(C) Linear regression between fold change of firing (burst frequency divided by baseline frequency) and inward current amplitude.

(D) Representative traces with mGluR1 antagonist MPEP (100 μM) and CPCCOEt (100 μM).

(E) Summary of inward current amplitudes with MPEP and CPCCOEt.

(F) Summary of the effect of TTX (200 nM) on inward current amplitudes in ChIs.

(G) Representative traces of dopamine input-driven inward currents in ChI with intracellular guanosine triphosphate (GTP) or GDPβS.

(H) Summary of the inward current amplitudes with GTP or GDPβS.

(I)Summary of inward current amplitudes with 0.1 mM EGTA and 10 mM BAPTA.

(J) Current-voltage (I-V) relationship of the dopamine-induced inward current.

(K) Representative traces and summary data in the presence of clemizole (10 μM).

(L) Summary of burst frequencies in M 084 (100 μM).

Error bars represent SEM. *p < 0.05, **p < 0.01.