Author response image 1. Photoheterotrophic growth of wild-type (WT) and M55 cells on BG-11 agar plates.
The WT and M55 mutant cells of Synechocystis were resuspended in BG-11 median at pH8.2. Three microliters of cell suspensions at OD750nm of 0.1 (top row), 0.01 (middle row), and 0.001 (bottom row) was spotted on agar plates. Five millimolars of Glucose and 10 μm DCMU were added to the plates for photoheterotrophic growth (right side) or were not added for photoautotrophic growth (left side). The plates were grown under ambient air for 8d at 50 μmol photons m-2 S-1.