Table 2. Strains and plasmids used in this study.
| Strain/genotype | Information | Source/reference |
|---|---|---|
| Wild-type (WT) | YBC16G1, prototrophic sigma1278b, MAT a | Isolate via Fink Lab |
| WT (pPCK1-mCherry) | Wild-type strain with gluconeogenesis reporter plasmid (mCherry with PCK1 promoter) | this study |
| WT (pHXK1-mCherry) |
Wild-type strain with constitutive reporter plasmid
(mCherry with HXK1 promoter) |
this study |
| WT (pTKL1-mCherry) | Wild-type strain with pentose phosphate pathway reporter plasmid (mCherry with TKL1 promoter) | this study |
| PCK1-flag | MAT a PCK1-3xFLAG::natNT2 | this study |
| FBP1-flag | MAT a FBP1-3xFLAG::natNT2 | this study |
| ICL1-flag | MAT a ICL1-3xFLAG::natNT2 | this study |
| MAL11-flag | MAT a MAL11-3xFLAG::natNT2 | this study |
| NTH1-flag | MAT a NTH1-3xFLAG::natNT2 | this study |
| ∆nth1 | MAT a nth1::kanMX6 | this study |
| ∆mal11 | MAT a mal11::kanMX6 | this study |
| ∆nth1 (pPCK1-mCherry) | ∆nth1 strain with gluconeogenesis reporter plasmid (mCherry with PCK1 promoter) | this study |
| ∆mal11 (pPCK1-mCherry) | ∆mal11 strain with gluconeogenesis reporter plasmid (mCherry with PCK1 promoter) | this study |
| ∆nth1 (pTKL1-mCherry) | ∆nth1 strain with pentose phosphate pathway reporter plasmid (mCherry with TKL1 promoter) | this study |
| ∆mal11 (pTKL1-mCherry) | ∆mal11 strain with pentose phosphate pathway reporter plasmid (mCherry with TKL1 promoter) | this study |
| Plasmid | Information | Source/reference |
| pPCK1-mCherry | mCherry under the PCK1 promoter and CYC1 termin- ator. p417 centromeric plasmid backbone, G418R. | this study |
| pHXK1-mCherry | mCherry under the HXK1 promoter and CYC1 termin- ator. p417 centromeric plasmid backbone, G418R. | this study |
| pTKL1-mCherry | mCherry under the TKL1 promoter and CYC1 termin- ator. p417 centromeric plasmid backbone, G418R. | this study |