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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Am J Transplant. 2019 Mar 29;19(8):2186–2198. doi: 10.1111/ajt.15313

Figure 3. Removal of CD8 from Treg cultures. (A) and (B) CD8, FoxP3 expression and in vitro suppressive capacity of two different Treg lines with comparable CD8 contamination and different FoxP3 expression.

Figure 3.

Treg lines from Figure 3A and 3B were expanded under protocol B. (C) CD8 single positive (SP) contamination on day 21 of culture between a Treg line that underwent MACS purification (left panel) and one that did not (right panel). (D) FoxP3 expression and suppressive capacity at the end of the culture period of Tregs that underwent CD8 MACS depletion prior to FACS sorting on day 0. (E) CD8 depletion by FACS early in culture to eliminate CD8 contamination. (F) Cell growth of a Treg line that underwent FACS on day 15 of culture to eliminate CD8 T cells. (G) FoxP3 and (H) suppressive capacity of a Treg line at the end of the culture period (day 56) after undergoing FACS on day 15 due to CD8 T cell contamination.