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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Am J Transplant. 2019 Mar 29;19(8):2186–2198. doi: 10.1111/ajt.15313

Figure 4. CD45RA expression in cultured Tregs and cryopreservation and recovery of Tregs under different conditions.

Figure 4.

(A) Correlation between FoxP3 and CD45RA expression in protocol B. (B) Correlation between FoxP3 and CD45RA expression in protocol D. (C) Average cell survival after 48 hours of culture post-thaw (n=3). Cell death was assessed by trypan blue and propidium iodide (PI). (D) FoxP3 expression after two days of culture under various conditions. (E) Average suppressive activity of freshly thawed Tregs and (F) 48 hours after culture (n=3).