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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Am J Transplant. 2019 Mar 29;19(8):2186–2198. doi: 10.1111/ajt.15313

Figure 6. Specificity study of in vitro expanded MCM Tregs and demethylation status of their TSDR.

Figure 6.

(A) Host, donor and third party PBMCs stimulated with anti-CD2CD3CD28 beads (n=3). Tregs were cultured following protocol B. Donor and third party PBMCs are full-MHC mismatched to host. Donor and third party PBMCs do not share any MHC. (B) Demethylation status of the TSDR of in vitro cultured Tregs grown under protocol A, B and D was analyzed in correlation with the FoxP3 expression and suppressive capacity of the same cell lines. (C) Study of the correlation between the TSDR demethylation status, FoxP3 expression and suppressive capacity of two Treg lines grown under protocol B over time.