Fig. 3. ALCAM deficiency attenuates T helper-dominant skin inflammation induced by epicutaneous OVA sensitization. (A) Mouse skin biopsy specimen stained with hematoxylin and eosin (×200, Scale bar = 50 μm) and scored according to histological features. (B) Serum levels of total IgE measured by enzyme-linked immunosorbent assay. (C-H) Skin mRNA levels were assessed by quantitative real-time polymerase chain reaction; (C) IL-4, (D) IL-5, (E) IL-13, (F) IFN-γ, (G) IL-17A and (H) IL-22.

The data represent mean ± standard error of the mean.

ALCAM, activated leukocyte cell adhesion molecule; NS, not significant; OVA, ovalbumin; PBS, phosphate-buffered saline; WT, wild-type; IgE, immunoglobulin E; IL, interleukin; IFN, interferon; mRNA, messenger RNA.

^*P < 0.05; ^†P < 0.01; ^‡P < 0.001 (n = 5–7 mice/group).