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. 2019 Jul 25;9:10842. doi: 10.1038/s41598-019-47132-5

Figure 6.

Figure 6

GPR39 is important for normal wound healing. (A) In situ hybridization analysis of skin wound samples from WT mice 3 days after injury. Skin samples were hybridized with a Gpr39-specific probe. Enlarged image of the boxed area is shown (upper right). The bar indicates 10 µm. (B) In situ hybridization and immunohistochemistry analysis of skin wound samples from WT mice 3 days after injury. Skin samples were hybridized with a Gpr39-specific probe. Skin fibroblast was then detected with anti-fibroblasts antibody (clone ER-TR7). The arrowhead indicates Gpr39 and fibroblast double-positive cell in the skin. The bar indicates 50 µm. (C) Real time-PCR analysis of skin fibroblast samples from WT mice 1 days after injury. The expression of Il-6 (left panel) and Gpr39 (right panel) were examined by real time-PCR analysis. Relative expressions were normalized against G3pdh. Data are means ± SD (n = 4 to 6). (D) Wound repair was evaluated in Gpr39−/− and control mice as in Fig. 2A. Values represent the mean + SEM. Filled circles, Gpr39−/− (n = 8 animals; female = 5, male = 3); filled squares, control (n = 10 animals; female = 6, male = 4). *P < 0.05, **P < 0.01 (two-tailed Student’s t-test), Gpr39−/− compared with control mice.