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. 2019 Jul 25;2:274. doi: 10.1038/s42003-019-0526-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — High-fat diets induced hepatic steatosis in adult and larval zebrafish. a Body weight of adult zebrafish fed with a control diet or high-fat diet (HFD) for 4 weeks. b Representative image of the abdominal subcutaneous fatty tissue histology with H&E staining. The scale bar is 100 μm. c Triacylglyceride (TAG) content in liver (n = 3, pool of three zebrafish per sample). d Representative liver histology image by H&E staining. The scale bar is 50 μm. e The expression of lipid metabolism-related genes in liver (n = 5, pool of three zebrafish per sample). f Representative image of whole-mount oil red O staining and H&E staining of liver sections in larvae fed control diet and high-fat diet. The scale bar is 100 μm. g The expression of lipid metabolism-related genes in zebrafish larvae. Gene expression was analyzed using cDNA prepared from pools of larvae in each group after seven days of feeding (n = 3, pool of 20 larvae per sample). Significance was established using a two-tailed student’s t-test. Differences are considered significant at P < 0.05 (*) and P < 0.01 (**)