Fig. 10.

The liver injury risks of common polysaccharides based on HIF1α. Germ-free (GF) zebrafish larvae fed sterile high-fat diet (HFD) for 1 week and immersed in gnotobiotic zebrafish medium contained 10 μg/ml different common polysaccharides for another 3 days. The expression of Hif-1αa (a) in GF larvae (n = 3, pool of 20 larvae per sample). b Serum AST in adult zebrafish fed on HFD, or HFD supplemented with a polysaccharide at 1.0% for 4 weeks (n = 3 or 4, pool of three zebrafish per sample). Data are expressed as the mean ± SEM. Significant increase compared with HFD is designated as P < 0.05 (*) and P < 0.01 (**). Significant reduction compared with HFD is designated as P < 0.05 (^#) and P < 0.01 (^##)