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. 2019 Jul 25;9:10783. doi: 10.1038/s41598-019-47246-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HRECs exposed to TNF-α, and high glucose for 24 h demonstrated decreased trans-endothelial electrical resistance. Representative ECIS tracings plotted as normalized resistance from all treatment groups (A). Representative confocal immunofluorescence images of the cells stained for VE-Cadherin. Boxed images shows higher magnification. White arrows indicate loss of cell-cell contacts (B). Data represent Mean ± SEM from 3 independent experiments performed in duplicates. **p < 0.01; *p < 0.05 compared to controls. Scale bar = 20 μm.