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. 2019 Jul 25;9:10783. doi: 10.1038/s41598-019-47246-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HRECs exposed to TNF-α, and high glucose for 24 h demonstrated alterations in VE-Cadherin and GRP78 association. Whole cell lysate were immunoprecipitated with antibody against VE‐Cadherin, and the immunocomplexes were analyzed by immunoblotting for GRP78 (A). Whole cell lysates were immunoprecipitated with complex partners (p-120 and β-catenin) and immunoblotted with GRP78 (B). β-tubulin served as an internal control. Data represent Mean ± SEM from 3 independent experiments performed in duplicates. ***p < 0.001; **p < 0.01.