Fig. 2.

Gap junction coupling between dispersing newly generated DGCs. a Schematic of the experimental setup, in which retrovirally labeled GFP-expressing newborn DGCs are whole-cell patch clamped and examined for reciprocal electrical coupling. b, d Representative current and voltage traces taken from two adjacent, electrically coupled, patched newborn DGCs at 5 dpi without b or with d 100 μM carbenoxolone treatment. The vertical scale bars are 100 pA/10 mV. Horizontal scale bar is 100 ms. c Representative image of biocytin-filled, retrovirally birth-dated 5-day-old DGCs from the experiment shown in panels b and d (left). Percentages of pairs of cells that were reciprocally electrically coupled (right). Scale bar is 10 μm (n = 4 mice). e Diagram and experimental timeline of the two-virus method of sparsely labeling clusters of newborn DGCs, in which lenti-GFAP-Cre and the FLEX-reverse GFP retrovirus were co-injected into the dentate gyrus. f Example of two cell clusters of GFP⁺ cells in the SGZ of the dentate gyrus using the two-virus method at 1 and 5 dpi, respectively. These sections have been stained with PCNA and GFAP for 1 dpi, and DCX and GFAP for 5 dpi. Scale bar is 10 μm. g Representative images showing biocytin-filled, retrovirally birth-dated 5-day-old DGCs from the experiment in e. Scale bar is 15 μm. h The number of pairs of cells that were reciprocally electrically coupled or non-coupled from the experiment in e (n = 3 mice)