Fig. 4.

Disruption of electrical coupling impairs integration of newly generated DGCs. a Experimental timeline of birth-date labeling and introduction of a dnCX43 into newborn DGCs. Representative images of control (GFP) and dnCX43-expressing newborn DGCs at 7 and 14 dpi (bottom). Scale bar is 20 μm. b Plots of numbers of apical dendrite branch points (left) and total lengths (right) at 5, 7, and 14 dpi for control and dnCX43-expressing DGCs. Branch points, each data point indicates the average of one animal, two-way ANOVA, 7 dpi, *P < 0.001; 14 dpi, *P < 0.001. Total length, each data point indicates the total length of one cell, two-way ANOVA, 7 dpi, *P < 0.001; 14 dpi, *P = 0.005 (n = 3–6 mice). c Table of electrophysiological properties of control and dnCX43⁺ cells at 5, 7, and 14 dpi. d, e Experimental timeline of birth-date labeling and introduction of dnCX43 into newborn DGCs from 4 or 7 dpi, with data collected at 14 dpi (top). Plots of branch numbers and total dendrite lengths at 14 dpi (data points shown similar as in b, bottom). Two-tailed unpaired t-tests, *P < 0.05, n.s. P > 0.05 (n = 3–5 mice). f Table of electrophysiological properties of control and dnCX43⁺ cells at 20–23 dpi. g, h In g, Sholl analysis of the dendritic tree of control and dnCX43⁺ at 21 dpi. In h, a summary of total dendritic length for control and dnCX43⁺ cells at 21 dpi (n = 25–38 neurons from three mice of each group. For g, statistical significance was determined by Student’s t-test; for h, ANOVA, *P < 0.05). i, j Glutamatergic synaptic transmission recorded from control and dnCX43⁺ DGCs at 21 dpi. i The percent of recorded newborn DGCs with detectable synaptic transmission on the left. On the right is eEPSCs amplitude. j Spontaneous glutamatergic synaptic transmission, frequency and amplitude (n = 7–12 neurons from three animals of each group; ANOVA, *P < 0.05). The value from each cell was used for statistical analysis and error bars represent SEM