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. 2019 Jul 25;10:3315. doi: 10.1038/s41467-019-11322-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Ion selectivity and kinetic properties of MerMAIDs a Representative photocurrent traces of MerMAID1 elicited with 500 nm light (gray bar) at different membrane potentials (−80 to +40 mV, in 20 mV steps, from bottom to top) before (left, gray) and after extracellular chloride reduction (right, cyan), as indicated. Insets show enlarged views of the remaining stationary photocurrent. b Current-voltage relationship of the MerMAID1 peak photocurrent at 150 mM (gray) and 10 mM (cyan) extracellular chloride ([Cl⁻]_e). Arrows indicate reversal potentials (E_rev). c Reversal potential shifts (ΔE_rev) upon reduction of [Cl⁻]_e for peak currents of all MerMAIDs as well as the stationary current of MerMAID1. d ΔE_rev values of MerMAID1 upon exchange of external buffer. ΔE_rev of the theoretical Nernst potential for Cl⁻ is indicated as a dashed line (c, d). e Extracellular pH (pH_e) dependence of biphasic MerMAID1 desensitization kinetic. Inset shows the time constants and their relative amplitudes to total decay. f pH_e dependency of the apparent desensitization time constant (τ_des) at −80 and +40 mV. g Voltage dependency of τ_des for all MerMAIDs in ms/mV. h Double-light pulse experiment at −60 mV and pH_e 7.2 to determine the peak current recovery time constant (τ_rec). i pH_e dependency of MerMAID1 at −60 mV. j Recovery time constants of all MerMAID variants. Mean values (thick lines) ± standard deviation (whiskers) are shown, and single-measurement data points are represented as dots. Source data are provided as a Source Data file (b–d, e–g, i, and j)