Fig. 4.

Differential occupancy of STAT3 and STAT1 at e4 and e5 enhancers. a Heatmaps showing STAT3 ChIP-seq signals at each enhancer cluster defined in Fig. 2a. b Boxplots depicting normalized tag counts at each enhancer cluster. ****p < 0.0001, paired-samples Wilcoxon signed-rank test. c Distribution of the average signal of STAT3 ChIP-seq at each enhancer cluster in LPS-stimulated (top) and IFN-γ-primed LPS-stimulated macrophages (bottom). d Representative UCSC Genome Browser tracks displaying normalized tag-density profiles at enhancers of IL10, TNIP3, IL4R, and IL23A in the four indicated conditions. Boxes enclose cluster e4 enhancer (blue) and cluster e5 enhancer (red). e Heatmaps showing STAT1 ChIP-seq signals at each enhancer cluster. f The boxplots indicate normalized tag counts at each enhancer cluster. g Distribution of the average signal of STAT1 ChIP-seq at each enhancer cluster in LPS-stimulated (top) and IFN-γ-primed LPS-stimulated macrophages (bottom). Data are representative of two independent experiments each of which included at least two independent donors (a–d) or are from GSE43036 (e–g). Boxes encompass the twenty-fifth to seventy-fifth percentile changes. Whiskers extend to the tenth and ninetieth percentiles. The central horizontal bar indicates the median