Fig. 7.

IFN-γ-mediated functional deactivation of STAT3-bound e4 enhancers. a Heatmaps of Pol II ChIP-seq signals at STAT3^hie4 and STAT3^loe4 enhancers (defined in Fig. 6a). The boxplots indicate normalized tag counts at STAT3^hie4 enhancers in the four indicated conditions (top) and at STAT3^hie4 and STAT3^loe4 enhancers in LPS-stimulated macrophages (bottom). ****p < 0.0001, paired-samples Wilcoxon signed-rank test. Boxes encompass the twenty-fifth to seventy-fifth percentile changes. Whiskers extend to the tenth and ninetieth percentiles. The central horizontal bar indicates the median. b Representative Genome Browser tracks showing RNA polymerase II (Pol II) occupancy, strand-specific RNA transcripts, and STAT3 occupancy (LPS condition) at enhancers of IL10. Boxes enclose HSS + 6 (left) and HSS-16 (right). c RT-qPCR analysis of enhancer RNA (eRNA) expression at two e4 enhancers (top, IL10-HSS + 6 and IL-10-HSS-16) and two e5 enhancers (bottom, IL6 and IL23A). Data are representative of three independent experiments. d RT–qPCR analysis of IL10 mRNA in resting and LPS-stimulated macrophages transfected with the indicated LNAs (IL10-HSS-16 eRNA and and IL10-HSS + 6 eRNA). Data are representative of two independent experiments