. 2019 Jul 19;10:813. doi: 10.3389/fphar.2019.00813

Table 1.

The inhibiting oligonucleotides and IC₅₀ values for hydrolysis of RNA target.

Inhibitor	Sequence, 5’-3’	IC₅₀, nM
r-inh	5’-r(CAAGCAGCCUACCC)	12 ± 4
m-inh	5’-C^mA^mA^mG^mC^mA^mG^mC^mC^mU^mA^mC^mC^mC^m	90 ± 30
d-inh	5’-d(CAAGCAGCCTACCC)	270 ± 90
pgd-inh1	5’-d(CxAxAxGxCxAxGxCxCxTxAxCxCxCx)-RNH₂	No effect
pgd-inh2	5’-d(CxAxAxGxCxAxGxCCTACCC)	400 ± 100
pgd-inh3	5’-d(CAAGCAGCxCxTxAxCxCxC)	100 ± 40

The cleavage conditions: 5 nM M1 RNA, 50 nM C5 protein, 100 nM fluorescent RNA target, 0-125 nM inhibiting oligonucleotide, 10 mM Tris-HCl, pH 7.5, 10 mM MgCl₂, 100 mM NH₄Cl, 37°C. N^m – 2’-O-methylribonucleotide; Nx – phosphoryl guanidine deoxyribonucleotide; x - phosphate groups modified with 1,3-dimethylimidazolidine-2-imine residues, RNH₂ = –(CH₂)₆NH₂. The results represent mean values (± SD) from two independent experiments.