Figure 6.

The NMT[G386E] Variant is Affected Differently by a Range of NMT Inhibitors

(A) Mean increase of EC₅₀ between PfNMT[G386E] and PfNMT[WT] parasites measured through SYBR Green growth assay (visualized on a log scale) (n = 2). A mean increase of 10 is indicated by a dotted line.

(B) Enzymatic CPM assay measuring the mean increase in IC₅₀ with different NMTi with purified PvNMT[WT] and PvNMT[G386E] (visualized on a log scale) (n = 3). A mean increase of 10 is indicated by a dotted line.

(C) Mean increase in binding affinity of NMTi for PvNMT[WT] and PvNMT[G386E] (visualized on a log scale) (n = 1–3). The K_D of each compound with PvNMT[G386E] was divided by the K_D with PvNMT[WT] to calculate the fold difference. K_D is the equilibrium dissociation constant of NMTi calculated from fitted response-concentration plots measured by SPR analyses. Only one K_D could be accurately determined for IMP-1002 and so error bars are not shown. A mean increase of 10 is indicated by a dotted line. All errors bars in this figure indicate standard deviation of the mean.