Figure 2.

Paraxial Mesodermal Cells Give Rise to the Cardiopulmonary, Subcutaneous, and Dermal Lymphatics

(A–A″) Immunofluorescence for tdTomato, PECAM, and PROX1 on a sagittal vibratome section at the level of the cardiac outflow tract from a Pax3^Cre/+;Rosa26^tdtomato embryo.

(B–B″) Immunofluorescence for tdTomato, PECAM, and PROX1 on a sagittal vibratome section at the level of the subcutaneous lymphatic network from a Pax3^Cre/+;Rosa26^tdtomato embryo.

(C–D′) Immunofluorescence for tdTomato, NRP2, and PECAM on whole-mount skin from Pax3^Cre/+;Rosa26^tdtomato embryos at the indicated positions and stages.

(Ao, aorta; Ot, outflow tract.)

Scale bars: 500 μm (A); 200 μm (B); 100 μm (A′ and A″); 50 μm (C); 20 μm (C′–D′).