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. 2019 Jul 25;9:10811. doi: 10.1038/s41598-019-46948-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of RIN1 on C2C12 myoblast differentiation. Structured illumination photomicrographs of C2C12 cells at 4 days under permissive differentiation conditions and drug treatment as indicated. Upper panels: Cells were stained for myosin heavy chain with the MF20 antibody (green) and labeled with DAPI to identify nuclei (blue) (upper panels). Lower panels: Cell body and nuclei image masks for quantification. (b,c) Image analysis, n = 3 wells, quantified the number of cell mask (green) objects (b) and the ratio of nuclei per cell mask object (c). Assay repeated 3 times.