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. 2019 Jul 25;9:10828. doi: 10.1038/s41598-019-47243-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment with Ex41 skip ASO increases ciliary localisation of membrane proteins AC3 and ARL13B in JBTS5 fibroblasts. (a) Representative immunofluorescence micrographs of control fibroblasts C1 and JBTS5 cells P1 and P2, serum starved for 48 h. Treatment of JBTS5 fibroblasts with 5 μM Ex41 skip ASO for 48 h increases AC3 ciliary localisation in JBTS5 lines P1 and P2. Green – AC3. Scale bar 5 μm. (b) Quantification of ciliary AC3 intensity after background subtraction. Values are expressed as AC3 ciliary intensity relative to ciliary intensity in C1 cells. *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA, n = 248. (c) Representative immunofluorescence images of control fibroblasts C1 and JBTS5 cells P1 and P2, serum starved for 48 h. Treatment of JBTS5 fibroblasts with 5 μM Ex41 skip ASO for 48 h increases ciliary localisation of the membrane protein ARL13B. Green – ARL13B. Scale bar 5 μm. (d) Quantification of ciliary ARL13B intensity normalised to the background. Values are expressed as ARL13B ciliary intensity relative to ciliary intensity in C1 cells. *P < 0.05, ***P < 0.001, one-way ANOVA, n = 281.

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